Advances and prospects in deuterium metabolic imaging (DMI): a systematic review of in vivo studies

Background Deuterium metabolic imaging (DMI) has emerged as a promising non-invasive technique for studying metabolism in vivo. This review aims to summarize the current developments and discuss the futures in DMI technique in vivo. Methods A systematic literature review was conducted based on the PRISMA 2020 statement by two authors. Specific technical details and potential applications of DMI in vivo were summarized, including strategies of deuterated metabolites detection, deuterium-labeled tracers and corresponding metabolic pathways in vivo, potential clinical applications, routes of tracer administration, quantitative evaluations of metabolisms, and spatial resolution. Results Of the 2,248 articles initially retrieved, 34 were finally included, highlighting 2 strategies for detecting deuterated metabolites: direct and indirect DMI. Various deuterated tracers (e.g., [6,6′-2H2]glucose, [2,2,2′-2H3]acetate) were utilized in DMI to detect and quantify different metabolic pathways such as glycolysis, tricarboxylic acid cycle, and fatty acid oxidation. The quantifications (e.g., lactate level, lactate/glutamine and glutamate ratio) hold promise for diagnosing malignancies and assessing early anti-tumor treatment responses. Tracers can be administered orally, intravenously, or intraperitoneally, either through bolus administration or continuous infusion. For metabolic quantification, both serial time point methods (including kinetic analysis and calculation of area under the curves) and single time point quantifications are viable. However, insufficient spatial resolution remains a major challenge in DMI (e.g., 3.3-mL spatial resolution with 10-min acquisition at 3 T). Conclusions Enhancing spatial resolution can facilitate the clinical translation of DMI. Furthermore, optimizing tracer synthesis, administration protocols, and quantification methodologies will further enhance their clinical applicability. Relevance statement Deuterium metabolic imaging, a promising non-invasive technique, is systematically discussed in this review for its current progression, limitations, and future directions in studying in vivo energetic metabolism, displaying a relevant clinical potential. Key points • Deuterium metabolic imaging (DMI) shows promise for studying in vivo energetic metabolism. • This review explores DMI’s current state, limits, and future research directions comprehensively. • The clinical translation of DMI is mainly impeded by limitations in spatial resolution. Graphical Abstract Supplementary Information The online version contains supplementary material available at 10.1186/s41747-024-00464-y.

Fluorine-18-fluorodeoxyglucose positron emission tomography is widely used in oncology for tumor staging, prognostic prediction, and treatment response evaluation [1,5,9,33,78].However, it is limited by radiation exposure (about 25 mSv for each examination) and incapability in monitoring downstream metabolism except for glucose intake [6,33].On the other hand, MRI and MRS, such as 1 H-MRS/MRSI and chemical shift saturation transfer, can monitor metabolism in vivo without exposing patients to ionizing radiation [30,36,54].However, these techniques can only be applied to observe the steady-state metabolism with complicated peak overlaps between different molecules (e.g., water, lipids) [54,70].To address these issues, a hyperpolarized MRI technique has been developed in the past decade [14,23,25,59,68,75].By utilizing the dynamic nuclear polarization technique, the MR signal of certain nuclei, such as 13 C, can be enhanced for more than 10,000 folds [26,51].Hyperpolarized MRI enables the visualization of metabolic pathways in real-time following the administration of exogenous hyperpolarized-13 C-labeled tracers (e.g., hyperpolarized- 13 C-pyruvate, hyperpolarized-13 C-fumarate) [23,26,59,75].However, the clinical translation of hyperpolarized MRI is slow due to the short-term enhanced signal (1-2 min) and the high cost of the equipment.DMI is a relatively new MR-based imaging technique that utilizes exogenous non-radioactive and biocompatible deuterium-labeled metabolic tracers such as [6,6′-2 H 2 ]glucose and [ 2 H 3 ]acetate to visualize different metabolic pathways in vivo [15,16,58].Compared to the aforementioned metabolic imaging techniques, DMI offers several distinct advantages including non-ionizing radiation, stable isotope labeling, biochemical safety, and relatively simple techniques [15,16,44,58].Compared with hyperpolarized- 13 C-MRI, DMI can be applied to observe a longer-term metabolic process (more than hours) [16].Although deuterium has a lower gyromagnetic ratio than the 1 H proton (6.536 MHz/T versus 42.577 MHz/T), it has a rapid longitudinal relaxation that enhances its detection [16,81].Unlike 1 H-MRSI, DMI does not require additional water suppression due to the low abundance of natural deuterated water (0.0115%, 10.12 mM), leading to a low specific absorption rate of radiofrequency power [15,16,44].Moreover, because of the reduced coupling effects of deuterium, the spectrum in DMI is much simpler to interpret than that of 1 H that has complex peak-splitting patterns [15,16].The combination of the low natural abundance of deuterium and the selective deuterium-labeling of metabolites by the tracer contribute to the further simplification of the spectrum in DMI.So far, many outstanding reviews or comments have been published on the applications of DMI [12,16,53,58,67,76,79,82]. To avoid repetition, this systematic review aims to summarize the current development of the DMI technique in vivo, outline present limitations, and discuss the potential research and development directions for the future.

Strategy of literature review
A systematic literature review was performed to summarize the deuterium metabolic MRI or spectroscopy in vivo based on the PRISMA 2020 statement [55].The PubMed database was used for the MeSH term search for previously published studies on deuterium metabolic MR imaging or spectroscopy from 1 January 2003 to 13 May 2023 (encompass earlier works that laid the foundation for subsequent advancements).The search strategy is described in Additional file 1: Table S1.A reference check was also performed.The inclusion criteria include the following: (1) original articles published with specific technique descriptions of MR imaging or spectroscopy involving deuterium detection to explore the metabolic processes in vivo and (2) studies were performed in living humans or animals, excluding studies performed on cell lines or isolated samples from humans or animals.Studies were selected for inclusion by two authors, who have 15 years of experience in diagnostic radiology and 15 years of experience in MR experiments.Decisions were made by consensus among all authors.

Data extraction and summarization
After conducting the literature review, a comprehensive extraction of specific technical details (including study subjects, examined body parts, target diseases/ specific physiological conditions, deuterated tracers, administration dose/route, and MR equipment/scanning parameters) was performed from the relevant articles.Additionally, potential applications, such as examination purposes and metabolic biomarkers, were collected.Two authors (FP and PS) performed the data extraction from all included studies.Subsequently, several key topics were summarized, including strategies of deuterated metabolites detection, deuterium-labeled tracers and corresponding metabolic pathways in vivo, potential clinical applications, routes of tracer administration, quantitative evaluations of metabolisms, and spatial resolution.

Results
The initial literature search resulted in a total of 2,248 articles.Following the screening process, 2,217 articles were excluded based on the inclusion criteria.Additionally, 3 articles were identified through citation searching.Finally, 34 published articles were involved (Table 1).The details of the database retrieval are shown in Fig. 1.

Strategies of deuterated metabolites detection: direct and indirect DMI
From the included articles (Table 1), detecting deuterium-labeled metabolites by DMI typically involves 2 strategies: direct (30 studies, including 25 animal studies and 6 human studies, with 1 study conducted in both animals and humans) and indirect (4 studies, including 3 human studies and 1 animal study) DMI.In the former strategy, 2 H-MRS/MRSI sequences were applied to selectively excite the deuterium nuclei and specifically capture the deuterium-labeled metabolite signals over time (Figs. 2 and 3) [15,62,65,73,81].In the latter strategy, the deuterium-labeled metabolites were not excited but detected indirectly through the corresponding 1 H-MR signal decrease, due to the chemical exchange between hydrogen and deuterium atoms following the administration of deuterium-labeled tracers (Fig. 4) [3,10,52,60].

Deuterium-labeled tracers and corresponding metabolic pathways in vivo
Deuterated tracers used in DMI are always the metabolites that are part of the cellular regular metabolism pathways [34].They contain deuterium atoms and can be metabolized like their non-labeled counterparts.When administered, these tracers can be incorporated into the corresponding metabolic pathways (e.g., glycolysis, TCA cycle, fatty acid oxidation) and metabolized into downstream deuterated compounds which can be detected and quantified by MRS/MRSI (Fig. 5) [34].Although a high-dose deuterium intake is harmful, the tracer doses widely employed in DMI, especially within the range of 0.6-0.8g/kg [6,6′-2 H2]glucose, have been extensively demonstrated to be non-toxic in vivo through previous studies [3,10,15,18,32,43,52,62,65].
In addition, some essential materials for cell proliferation can also be deuterated as tracers in DMI, such as [ 2 H9]choline and heavy water (Fig. 5) [8,31,72].Choline is a precursor for phosphatidylcholine, a major component of cell membranes produced in the Kennedy pathway.By using [ 2 H9]choline as a tracer, DMI can image and quantify the total pool of choline in vivo, which can reflect the cellular proliferation activity [31,72].Exogenous heavy water administrated into the body water pool can also be used as a tracer, which can be incorporated into various macromolecules such as lipids, proteins, and deoxyribonucleic acid [7,8,20,42].However, most synthesized molecules such as lipids and proteins will gradually lose deuterium labels over time owing to the energy metabolism except de novo deoxyribonucleic acid; thus, measuring long-term deuterium enrichments can help to quantify the de novo deoxyribonucleic acid which can serve as a proxy indicator for cell proliferation [7,8,20].
Moreover, another tracer type is the non-metabolizable analog of metabolic precursors.For example, deuterated 3-O-methylglucose is a glucose analog that is taken up by cells but not metabolized further, thus specifically reflecting the glucose uptake [27].
DMI has also presented great potential in estimating residual tumors and early response after various anticancer treatments (e.g., chemotherapy, targeted therapy, radiotherapy) (Table 2) [2,15,24,28,37,69].From previous studies, DMI presented a higher sensitivity in identifying tumoral necrosis and residue than conventional imaging techniques that mainly rely on morphological changes [2,15,24,28,37,69].A necrotized tumor after treatments first demonstrated a significantly declined Warburg effect, accompanied by a recovered TCA cycle activity and suppressed glycolysis, before the reduction of glucose intake and following morphological necrosis [15,24,37,69].Thus, DMI can also be applied in the early treatment-response evaluation with a potentially higher sensitivity than conventional methods; for example, previous studies revealed that early tumoral necrosis presented a significantly increased fumarate/malate conversion rate (recovered TCA cycle activity) after administrating [2,3-2 H2]fumarate in DMI [28,75].
Moreover, some animal studies found that DMI can also reflect various metabolic abnormalities under other diseases or physiological conditions, such as chronic graft-versus-host disease after hematopoietic stem cell transplantation, preeclampsia, ischemic stroke, cold acclimation, deep anesthesia, and analgesia condition (Table 2) [8,44,48,50,61].Although these experiments were performed on animals, they can also be extended to human beings [8,44,48,50,61].

Routes of tracer administration: oral, intravenous bolus/ infusion, and intraperitoneal
In DMI, deuterated tracers can be administered in several ways (Table 3): oral intake, intravenous (IV) bolus injection, IV infusion, intraperitoneal (IP) bolus injection, IP [ 2 H9]choline [31] Total choline uptake Choline is a component of phospholipids, which are essential for the formation and maintenance of cell membranes.Tumor cells have increased choline uptake and metabolism due to their high demand for membrane synthesis and growth.Thus, the malignant tumor presents a higher choline uptake than normal tissue.

Explanations
Rehabilitation evaluation Ischemic stroke [50] [6,6′-2 H2]glucose [50] Lactate and Glx synthesis After ischemic stroke, normal oxidative metabolism is replaced by glycolysis resulting in cerebral lactate accumulation and Glx depletion.Monitoring the changes of lactate and Glx levels in the stroke region can help to evaluate cerebral recovery over time.

Specific physiological conditions
Brown adipose tissue activity under cold acclimation [61] [6,6′-2 H2]glucose [61] Glucose uptake Activated brown adipose tissue is essential to combat obesity and metabolic syndrome in vivo, because it brings an increased energy expenditure.Thus, activated brown adipose tissue has a significantly higher glucose uptake.
DMI Deuterium metabolic imaging, Glx Glutamine and glutamate, TCA Tricarboxylic acid, tCho Total choline pool, TERT Telomerase reverse transcriptase, PARP Poly adenosine diphosphate-ribose polymerase infusion, applying a bolus variable infusion protocol, and IV bolus injection followed by oral intake.Oral administration is the only attempted administration route in humans so far, but it may result in delayed or variable tracer absorption, depending on a range of varying factors (e.g., food intake, gastric pH), leading to unstable kinetics [63].In contrast, IV administration allows for more precise control of the dose and timing of the tracer than oral intake, ensuring uniform distributions of the tracer throughout the body and benefiting a more precise kinetic quantification [63].Nevertheless, a rapid IV bolus administration might affect the physiology of the body (e.g., changes in blood pressure, heart rate) and alter the intracellular metabolic states in some cases by competing receptors, transporters, and enzymes [31,72,77].Instead, an IV infusion protocol reduces these adverse effects and is particularly useful when studying steady-state metabolism [15,17].Still, this infusion protocol has some issues that need to be considered, one of which is the relatively longer time required to reach a real steady-state concentration of the tracer [31,49,60].To achieve a steady state faster, a bolus variable infusion protocol was developed involving a bolus injection of the tracer, followed by a slow but constant infusion [31,49,60].This method may improve the stability and linearity of metabolic quantification during a more than 1-h imaging period [31,49,60].Yet, the bolus variable infusion protocol has challenges that should be considered, such as optimizing variable infusion parameters (e.g., dose, duration, infusion rate).IP administration is another convenient way when performing animal studies [15,17,74,81].The technique of IP injection is easier than that of IV administration.It allows for the direct delivery of the tracer to the blood circulation through peritoneal absorption.However, this route is probably useless in humans because of the potential risk of bowel injury.Like IV administration, IP administration can also be implemented via a bolus injection or continuous infusion [15,17,74,81].

Quantitative evaluations of metabolic processes
Before the metabolic quantification, various corrections and normalization procedures should be implemented to obtain accurate molar concentrations of the tracer and metabolites in DMI: 1) Correction for signal variations due to incomplete longitudinal relaxation and RF transmit B 1 + magnetic field inhomogeneities (the actual RF flip angles) based on measured T1 relaxation times for various

Table 3 Different administration routes of deuterated tracers in vivo
NA Not applicable, IP Intraperitoneal, IV Intravenous a There is an exception in that one study reported an 8-min IV infusion [74] b Briefly, it is a three-step bolus-continuous infusion protocol, in which animals received an IV bolus injection of the deuterated tracer (about one-sixth of the total dose) over 15 s, followed by a gradual reduction of infusion until a constant infusion rate was reached for the remainder of the experiment [31,60] c Briefly, an initial IP bolus injection of 35 mL/kg 2 H 2 O was performed, followed by continuous administration of 8% (volume/volume) 2 H 2 O in drinking water during specified labeling periods [8,20]
Besides, there are two other accessible quantification methods: (1) Calculation of the area under the curves of target metabolites over time [24,28,29,61,69,73].A previous study for hyperpolarized-MRI demonstrated the significantly improved reliability of area under the curves-based analysis compared to multiple kinetic analysis, which may shed light as well on the data analysis for DMI [13].(2) Single time point quantifications [15,18,31,32,41,43,50,65,72,81].Instead of focusing on dynamic metabolic processes, this method only measured the relative abundance of deuterium-labeled metabolites at a particular time point (commonly after 1 h) [15,18,31,32,41,43,50,65,72,81].Although it does not provide dynamic information about specific metabolisms, it has been proven as a feasible way to diagnose malignant tumors and evaluate early anti-tumor treatment responses in humans [15].Compared to kinetic analysis, these two quantification methods have three advantages: simplicity, robustness, and practicability.

Spatial resolution
The clinical transformation of DMI faces one major challenge: an insufficient spatial resolution with a low signal-to-noise ratio (SNR) [18] (Table 4).Despite the administration of exogenous deuterium-labeled tracers, the concentration of deuterium is still low leading to weak signals.This contrasts with hyperpolarized- 13 C-MRI which provides a higher spatial resolution (0.05 mL versus 0.21 mL) with a shorter acquisition time (6 s versus 60 s) at 4.7 T than DMI [73].In a study exploring DMI at 3 T, the spatial resolution reached 3.3 mL with an acquisition time of 10 min [32].If fixing the acquisition time, spatial resolution and SNR can be improved directly by increasing the B 0 intensity [18].A previous study has shown the SNR increased from 169.2 ± 13.3 at 4 T to 423.1 ± 25.7 at 7 T [18].In animal MR scanners above 9 T, the spatial resolution can reach 0.1 mL with an acquisition time of 5-10 min (Table 4).However, high-field magnet cost and safety issues (e.g., higher radiofrequency energy deposition, dizziness, nausea) limit the clinical translation of DMI [39].Presently, only 7-T MRI equipment is validated for DMI in clinics, offering a spatial resolution of approximately 1 mL; conversely, this comes with the trade-off of an acquisition time of approximately 30 min (Table 4 and Additional file 1: Table S2) [18].Although increasing acquisition time is the easiest way to increase spatial resolution by accumulating sufficient signals (Table 4), it introduces the risk of motion artifacts and patient discomfort during the examination.Interpolation and zerofilling are commonly used to enhance spatial resolution, but these enhancements are fake [44,47].
Another way to address the spatial resolution or SNR issue in DMI is to improve imaging sequences and coils.The implementation of an Ernst-angle approach, considering an assumed or measured in vivo T1, has been shown to enhance signals effectively; for instance, employing a flip angle of 68° for breast cancer in rats with an in vivo measured T1 of 250 ms has demonstrated promising results (Table 4) [3,8,27,62].Another study proposed a multi-echo balanced steady-state free precession approach to enhance SNR (Table 4) [56].Compared to conventional chemical shift imaging (repetition time/ flip-angle = 95ms/90°), the multi-echo balanced steadystate free precession imaging (echo time 2.2 ms, repetition time 12 ms, flip angle 60°) demonstrated a predicted SNR increase of three to five times with matched spatial resolution and scan time, while maintaining good agreement in the time courses of all metabolites [56].In a previous study, a dual-tuned array coil was introduced, featuring ten transmitting/receiving channels for 1 H and eight transmitting/two receiving channels for 2 H, paired with an Ernst-angle three-dimensional chemical shift imaging sequence [62].This configuration provided a nominal spatial resolution of 0.003 mL with an acquisition time of 10 min and achieved a successful DMI implementation in humans at 7 T (Table 4) [62].

Discussion
In this work, we provide an extensive overview of the current development of the DMI technique.By analyzing 34 published articles, we introduced and summarized specific technical details and potential applications of DMI in vivo.In the following part, we will discuss present limitations, potential research, and development directions of DMI for the future.So far, direct DMI is still the mainstream technique.While the SNR is constrained by the low gyromagnetic ratio of the deuterium isotope, this limitation is offset by the relatively short T1 of the deuterium-labeled tracer [16,81].This characteristic facilitates rapid signal acquisition and allows for a large number of excitations without a significant signal saturation [16,81].Compared with direct DMI, indirect DMI has a reported five times higher SNR and can even be performed at commercial 3-T MRI scanners [52,60].This eliminates the need for extra spectrometers, specialized deuterium coils, and dedicated MRI sequences.However, from a previous study, the correlation between indirect and direct metabolite quantification was not high (r = 0.62) [60].It indicates the 1 H-MR signal reduction might be affected by several factors, such as tracer administration methods, stress reaction after tracer administration, participant movement (e.g., participants moved in and out of the scanner to drink the tracer), and so on [31,72,77].After all, the basic hypothesis of indirect DMI is the difference in the absolute metabolite concentrations over time at each pixel.If the absolute metabolite concentrations were affected by tracer administration, the signal reduction would not indicate the real deuterium-labeled metabolite concentrations.Thus, we propose exploring methods to maintain intra-environmental stability of metabolism to address these concerns.
In some cases, the deuteration of certain molecules may affect their metabolism, such as cytochrome P450-mediated oxidative metabolism [4,64].Especially, it is essential to address safety considerations in the development of analog tracers [27].After all, intracellular accumulation of the analog might inhibit glycolysis and bring potential toxicity [38,80].However, the deuterated tracers reported in Table 5 rarely interrupt the natural metabolism at the indicated doses and can be safely used in vivo [2, 3, 10, 15, 17, 18, 24, 28, 29, 31, 32, 37, 41, 43, 44, 46-48, 50, 52, 56, 60-62, 65, 66, 69, 72-74, 81, 83].With the help of these different tracers, we can understand the metabolic status of various tissues and organs in living bodies and find the metabolic alterations in various diseases, such as cancer, preeclampsia, and neurological disorders [15,34,48,50].New development of tracers is still on the way; it is worthwhile to develop tracers on more specific metabolic pathways while prioritizing safety and minimizing label loss.
Overall, DMI holds potential for various medical applications in the future, including cancer diagnosis, early response evaluation after anti-tumor treatment, nutritional studies, and metabolic disease research.As another

Table 5 A summary of deuterated tracers used in vivo
Glu4 [4,4′-2 H2]glutamate and [4′-2 H]glutamate, Glx Glutamine and glutamate, TCA Tricarboxylic acid, tCho Total choline pool a Instead of a single Glx peak in direct DMI, individual distinction of Glu4 from glutamine can be achieved by indirect DMI as mentioned above [60] b Deuterated choline cannot be identified from its downstream metabolites such as phosphocholine and glycerophosphocholine under magnetic resonance spectroscopy because of the spectral overlap; thus, the deuterium-labeled pool of choline plus metabolites was quantified c No chemical shift imaging or spectroscopy was performed in this study; instead, non-selective deuterium-nuclear MRI was applied with the dual transmit/receive coil tuned to 61. 45  deduction that merits further exploration, DMI might also help identify the "pseudoprogression" after immune checkpoint inhibitor therapies, characterized by an initial increase followed by a subsequent decrease in the size of existing tumors after treatment [22,40].In addition, due to the non-radioactive feature, DMI presents a potential utilization in human fetuses and pregnant women, although definitive evidence is yet to be established; however, the feasibility of DMI has been demonstrated in pregnant mice [48].
There are different administration routes in DMI, but the comparisons between varied routes in vivo are still limited.After all, the different routes of administration may affect the plasma glucose level and the deuterium enrichment of downstream metabolites, which can influence the sensitivity and accuracy of DMI.One previous study explored the discrepancies between IV and IP infusions of [6,6′-2 H2]glucose and found that IV infusions caused the glucose signal to rapidly reach a plateau in the liver, while IP infusions showed a continuous rise in the glucose signal, far surpassing the water signal [17].Another study comparing IV bolus injection and infusion in mice found that while infusion led to a smoother glucose response without an initial spike, minimal differences were observed in downstream lactate metabolite quantification compared to bolus injection [48].So far, while the emphasis on oral administration for humans has stemmed from its perceived safety, simplicity, and well-tolerance, alternative administration routes have not been extensively explored rather than being deemed inherently infeasible.Therefore, for clinical translation, it is essential to comprehensively compare different tracer administration routes in further investigations.
Although kinetic analysis is the most commonly applied quantification in DMI, it still has several challenges.First, it requires multiple time point measurements, which can be time-consuming and resource-intensive.Second, it requires complex mathematical modeling to calculate kinetic parameters, requiring a sufficiently short acquisition time of DMI.Third, even the infusion kinetic analysis may lead to inaccurate estimation of metabolic rates because the tracer is always compartmentalized within specific tissues or metabolic pathways resulting in a very complex kinetic system [3,8,27,60,74].To tackle these issues, quantification methods, including calculation of the area under the curves of target metabolites over time and single time-point quantifications, can be considered [15,18,24,28,29,31,32,41,43,50,61,65,69,72,73,81].However, these two methods are limited because they may not capture the dynamic information as effectively as kinetic modeling.Overall, future research is likely to focus on addressing the challenges associated with kinetic analysis while exploring alternative quantification methods to enable more accurate and efficient assessment of metabolic processes in clinical scenarios.
To date, the clinical transformation of DMI heavily hinges on addressing the limitation of spatial resolution and SNR.Enhancing deuterium enrichment of tracers (e.g., [2,3,4,6,6′-2 H5]glucose and [ 2 H7]glucose) and applying the indirect DMI strategy are both potentially applicable to enhance SNR for DMI, as introduced in previous sections (Table 4 and Additional file 1: Table S2), but these advancements have yet to fully overcome existing challenges [3,46,52,60,83].Recently, deep learning techniques have offered a promising avenue for DMI denoising, resulting in improved SNR.In a pioneering study, researchers proposed a novel machine learning-based approach that synergistically integrates physics-based subspace modeling and data-driven deep learning to achieve effective denoising, enabling high-resolution DMI [41].In addition, a less explored technique in DMI is compressed sensing, which may hold the potential to reduce the acquisition time while preserving data quality by leveraging the inherent sparsity of metabolic signals in a relatively wide spectrum, meriting further investigations [19,21].
In conclusion, DMI holds promise for improving clinical diagnostics and treatment protocols by offering new insights into metabolic disorders and diseases.Despite significant advancements, limitations in spatial resolution still hinder the clinical translation of DMI techniques.Additionally, to unlock the full clinical potential of DMI, it is essential to optimize tracer synthesis, administration protocols, and quantitative analysis methodologies.
expertise and assistance in the paper writing.The authors declared LLMs were not used.

Fig. 1
Fig. 1 PRISMA 2020 flow diagram of the retrieved database

Fig. 2 AFig. 3 AFig. 4 Fig. 5
Fig. 2 A representative 2 H-MRS obtained from the liquid cultured medium for a tumor cell line (MC38 murine colon adenocarcinoma) after being exposed to [6,6′-2 H2]glucose at different time points (a).Over time, the 2 H-labeled glucose level gradually decreased but the 2 H-labeled lactate gradually increased indicating a typical Warburg effect of the malignant cell line (b).Figures were provided by author QB.All experiment was performed in Bruker 500-MHz MR scanner

Table 1
The 34 included articles

Table 1 (continued) First author [reference number] Year Deuterated tracers Target living subjects in the study Examined body parts by DMI Target diseases or specific physiological conditions MR equipment
a DMI Deuterium metabolic imaging, MR Magnetic resonance a Direct DMI was performed in the study b Indirect DMI was performed in the study

Table 2
Potential applications of DMI in vivo

Table 4
A summary of spatial resolution among different studies The Point RESolved Spectroscopy sequence was applied instead of the free induction decay sequence g Interleaved DMI was implemented within the fluid-attenuated inversion recovery acquisition period f

Deuterated tracers Targeted metabolic pathways Detected deuterium-labeled metabolites and corresponding chemical shift in vivo (ppm)
MHz [8]